We have investigated the level of antioxidants
(glutathione (GSH), GSH-reductase, GSH-peroxidase, GSHtransferase,
catalase (CAT) and superoxide dismutase (SOD)),
lipid peroxidation products (plasma, erythrocyte and urinary
malondialdehyde (MDA)) and urinary DNA damage product
8-hydroxydeoxyguanosine (8-OH-2’-dG) in metal-shelf factory
workers and compared the levels to those of a control group working
in a different work area.
Methods: Twenty four workers working in a metal shelf
production factory and sixteen apparently healthy male volunteers
without environmental and work place exposure were included in the
study. Blood and urine samples were examined for oxidative stress
parameters. Measurement of 8-OH-2’-dG was by high performance
liquid chromatography (HPLC), while other parameters were
measured by colorimetric methods.
Results: GSH-transferase, CAT, SOD activities and plasma,
erythrocyte and urinary MDA levels of metal shelf production
factory workers were higher than for the control group, whereas,
GSH-peroxidase and GSH in workers were lower than for controls.
Moreover, while GSH-reductase activity was lower, 8-OH-2’-dG
level was higher than the control group but the difference was not
Conclusion: As a result of increased industrialization, air
pollution and hazardous working conditions there is a deterioration
of the balance between oxidant and antioxidant levels which affects
the structure of macromolecules such as DNA, lipids and proteins.